In building the tanks themselves, regard must be had to their special purposes. If intended for show-tanks for popular admiration, or for the study of large animals, they must be large with a plate-glass front; for ordinary scientific work small tanks with all sides opaque are preferable from every point of view. According to their character, size and position, fixed tanks may be of brickwork, masonry or rock, coated in each case with cement; asphalting the sides offers no particular advantages, and often gives rise to great trouble and expense. All materials, and especially the cements, must be of the finest quality procurable. For smaller and movable tanks, slate slabs bolted or screwed together have some disadvantages, notably those of expense, weight and brittleness, but are often used. Better, cheaper and lighter, if less permanent, are tanks of wood bolted together, pitched internally. Glass bell-jars, useful in particular cases, should generally have their sides darkened, except when required for observation. Provision should always be made for cleaning every part of the tanks, pipes and reservoirs; all rock-work in tanks should therefore be removable. As regards the lighting of fixed tanks, it should always be directly from above. In all tanks with glass sides, whether large or small, as much light as possible should be kept from entering through the glass; otherwise, with a side-light, many animals become restless, and wear themselves out against the glass, affected by even so little light as comes through an opposite tank.
In cases where distance from the sea or other causes make it impracticable to allow the overflow from the tanks to run to waste, special precautions must be taken to keep the water pure. Chemically speaking, the chief character of the water in an aquarium circulation, when compared with that of the open sea, lies in the excessive quantity of nitrogen present in various forms, and the reduced alkalinity; these two being probably connected. The excess of nitrogen is referable to dead animals, to waste food and to the excreta of the living organisms. The first two of these sources of contamination may be reduced by care and cleanliness, and by the maintenance of a flow of water sufficient to prevent the excessive accumulation of sediment in the tanks. The following experiment shows the rapid rise of nitrogen if unchecked. A tank with a considerable fauna was isolated from the general circulation and aerated by four air-jets, except during hours 124-166 of the experiment; column I. shows per 100,000 the nitrogen estimated as ammonia, column II. the total inorganic nitrogen:--