The 1995–96 season was the 81st season of the Isthmian League, which is an English football competition featuring semi-professional and amateur clubs from London, East and South East England. The league consisted of four divisions.
Premier Division
The Premier Division consisted of 22 clubs, including 18 clubs from the previous season and four new clubs: Worthing, promoted as runners-up in Division One Boreham Wood, promoted as champions of Division One Chertsey Town, promoted as third in Division One Yeovil Town, relegated from the Football Conference
League table
Division One
Division One consisted of 22 clubs, including 15 clubs from the previous season and seven new clubs:
Three clubs relegated from the Premier Division: Chesham United Marlow Wokingham Town
Three clubs promoted from Division Two: Barton Rovers Oxford City Thame United
Plus: Leyton Pennant replaced Leyton, who merged with Walthamstow Pennant.
League table
Division Two
Division Two consisted of 21 clubs, including 16 clubs from the previous season and five new clubs:
Two clubs relegated from Division One: Wivenhoe Town Dorking
Three clubs promoted from Division Three: Canvey Island Collier Row Bedford Town
Division started the season one club short after Newbury Town folded. At the end of the season Saffron Walden Town resigned from the league due to ground grading problems and joined the Essex Senior League. Thus, no teams were relegated from the Second Division this season.
League table
Division Three
Division Three consisted of 21 clubs, including 17 clubs from the previous season and four new teams:
Two clubs relegated from Division Two: Aveley Windsor & Eton
Plus: Wealdstone, transferred from Southern League Southern Division Wingate & Finchley, promoted as runners-up in the South Midlands League