Gregarina garnhami is a eukaryotic unicellular organism belonging to the Apicomplexa described in 1956 by Canning as a parasite found in several locusts, such as the desert locust, African migratory locust, and Egyptian locust. Especially, the desert locust is the host for this species, as up to 100% of animals can become infected. An estimated thousands of different species of gregarines can be in insects and 99% of these gregarines still need to be described. Each insect is said to host multiple species. A remarkable feature of G. garnhami is its autofluorescence.
Taxonomy
Gregarina garnhami was considered by Lipa et al. in 1996 to be synonymous with Gregarina acridiorum (Léger, 1893), a parasite of several orthopteran species including Locusta migratoria. Indeed, G. acridiorum and G. garnhami share common morphological and behavioral characteristics, such as their development in the midgut of their hosts, a small globular epimerite, stout bodied gamonts, and barrel-shaped (or dolioform) oocysts. In 2021, an integrative taxonomy study, using morphological and molecular characters, concluded that the two species were distinct and thus confirmed the validity of G. garnhami. However, phenotypic plasticity was clearly observed in the case of G. garnhami: the morphology of its trophozoites, gamonts and syzygies varied according to the geographical location of S. gregaria and the subspecies infected.
Cell structure
Gregarina garnhami is a gregarine that belongs to the septate eugregarines, meaning its cell is separated into parts. In G. garnhami, three parts can be seen: epimerite, protomerite, and deutomerite, but their visibility depends on the lifestage of the organism. A characteristic of gregarines is the typical construction of the pellicula that is formed by a cell membrane and two cytoplasmic membranes (the latter is often referred to as the inner membrane complex, IMC or alveoli). The membranes' proximity to one another often makes them difficult to distinguish. This structure is often also referred to as the trilayered structure. Beneath the inner membrane, a basal (internal) lamina can be seen, which separates the foldings around the cell from the rest of the cell. These foldings form the outer surface of G. garnhami, and hundreds of these can be observed at the surface enlarging the surface of the cell. The cytoplasm of the cell (in the epimerite, protomerite, and deutomerite) is divided in two zones: ectoplasm and endoplasm. The ectoplasm is clear and does not contain much granular material. In the ectoplasm, the microtubules can be found. The endoplasm is less transparent and contains paraglycogen giving the cells a brown-yellowish color when viewing the cells with a light miscrope.
Life cycle
The lifecycle consists of several stadia: gametocyst - oocyst - sporozoite – trophozoite (chepaline) - gamont (gametocyte, sporadin) – gametocyst – oocyst. The gametocyst and oocysts are the cell structures that can survive outside the host-organism and infect other insects. The cycle starts with the oocysts that leave the body with the feces and are left on plant-material. When other locust than eat the plant with oocysts they will burst open (excystation) under the influence of the digestion of the locust. Eight sporozoites will then be released inside the digestive system of the locust.<ref>{ { cite journal |last1=Janardanan |first1=K.P. |last2=Ramachandran |first2=P. |title=On a new septate gregarine, Stenoductus polydesmi' sp. n. (Sporozoa: Cephalina) from the Millipede, Polydesmus sp. with observations on host-specificity of gregarines infecting the millipedes in Kerala, India |journal=Acta Protozoologica |date=1983 |volume=22 |issue=1 |pages=87–94}}</ref> Orthoptera regurgitate enzymes from the foregut and together with secretions of the salivary glands these enzymes can break down the oocyst cell wall and thus enable the freeing of the sporozoits. The free sporozoits than pass through the peritrophic membrane surrounding the midgut. Once in the ectoperitrophic space they can attach themselves to the epithelial cells of the caeca and midgut.