John Best may refer to:
Politicians
John Best (died 1560), MP for Westminster
John Best (died 1574), in 1553 MP for Colchester
John Best (British politician) (1821–1865), British barrister and politician
John Best (Canadian politician) (1861–1923), Australian-born politician in Canada
Sports
John Best (basketball) (born 1971), American basketball player
Johnny Best (boxer), 1940s Australian middleweight boxer
John Best (soccer) (1940–2014), US/English soccer defender
John Orr Best (1910–1996), American soccer referee and member of the National Soccer Hall of Fame