Kowalewsky (_Embryo. Stud. an Vermen u. Arthropoda_, Mem. Akad. St Petersbourg, 1871) finds that the segmental tubes of Annelids develop from the mesoblast. We must therefore look upon the mesoblastic origin of the excretory system as having an antiquity greater even than that of vertebrates.
VIII. ON THE DEVELOPMENT OF THE SPINAL NERVES IN ELASMOBRANCH FISHES[47].
Footnote 47: [From the _Philosophical Transactions of the Royal Society of London_, Vol. CLXVI. Pt. 1. Received _October 5_, Read _December 16, 1875_.]
In the course of an inquiry into the development of Elasmobranch Fishes, my attention has recently been specially directed to the first appearance and early stages of the spinal nerves, and I have been led to results which differ so materially from those of former investigators, that I venture at once to lay them before the Society. I have employed in my investigations embryos of _Scyllium canicula_, _Scyllium stellare_, _Pristiurus_, and _Torpedo_. The embryos of the latter animal, especially those hardened in osmic acid, have proved by far the most favourable for my purpose, though, as will be seen from the sequel, I have been able to confirm the majority of my conclusions on embryos of all the above-mentioned genera.
A great part of my work was done at the Zoological Station founded by Dr Dohrn at Naples; and I have to thank both Dr Dohrn and Dr Eisig for the uniformly obliging manner in which they have met my requirements for investigation. I have more recently been able to fill up a number of lacunæ in my observations by the study of embryos bred in the Brighton Aquarium; for these I am indebted to the liberality of Mr Lee and the directors of that institution.
_The first appearance of the Spinal Nerves in Pristiurus._
In a _Pristiurus_-embryo, at the time when two visceral clefts become visible from the exterior (though there are as yet no openings from without into the throat), a transverse section through the dorsal region exhibits the following features (Pl. 22, fig. A):--
The external epiblast is formed of a single row of flattened elongated cells. Vertically above the neural canal the cells of this layer are more columnar, and form the rudiment of the primitively continuous dorsal fin.