The 72nd edition of the KNVB Cup started on September 2, 1989. The final was played on April 25, 1990: PSV beat Vitesse Arnhem 1–0 and won the cup for the sixth time.
Teams
All 18 participants of the Eredivisie 1988-89, eight of which entering in the second round All 19 participants of the Eerste Divisie 1988-89 27 teams from lower (amateur) leagues
First round
The matches of the first round were played on September 2 and 3, 1989.
E Eredivisie; 1 Eerste Divisie; A Amateur teams
Intermediary Round
There was only room for 32 teams in the next round, so this intermediary round was held on October 4, 1989.
Second round
The matches of the second round were played on December 8, 9, 10 and 13 1989. The ten highest ranked Eredivisie teams from last season entered the tournament this round.
E ten Eredivisie entrants
Round of 16
The matches of the round of 16 were played on January 13 and 14, 1990.
Quarter finals
The quarter finals were played on February 14, 1990.
Semi-finals
The semi-finals were played on March 14, 1990.
Final
PSV would participate in the Cup Winners' Cup.