First-seeded Margaret Smith defeated Maria Bueno 5–7, 6–1, 6–2 in the final to win the women's singles tennis title at the 1964 French Championships.
Seeds
The seeded players are listed below. Margaret Smith is the champion; others show the round in which they were eliminated.
Margaret Smith (champion) Maria Bueno (finalist) Lesley Turner (semifinals) Nancy Richey (fourth round) Jan Lehane (quarterfinals) Christine Truman (quarterfinals) Françoise Dürr (second round) Věra Suková (quarterfinals) Deidre Catt (fourth round) Annette Van Zyl (fourth round) Robyn Ebbern (fourth round) Liz Starkie (third round) Helga Schultze (semifinals) Madonna Schacht (fourth round) Judy Tegart (fourth round) Lea Pericoli (fourth round)