These preventives only prove efficient on condition that they be used persistently. Let our vigilance be lacking for an instant, and the enemy will enter to work destruction, for it only requires a spore less than a hundredth of a millimeter in diameter to produce the most serious affections.
Fortunately, and it is again to Mr. Pasteur that we owe these wonderful discoveries, the parasitic microbes themselves, which sow sickness and death, may, through proper culture, become true vaccine viri that are capable of preserving the organism against any future attack of the disease that they were capable of producing; such vaccine matters have been discovered for charbon, chicken cholera, the measles of swine, etc.
When the _micrococcus_ of chicken cholera (Fig. 3.) is cultivated, it is seen that the activity of the microbe in cultures exposed to the air gradually diminishes. While a drop of the liquid would, in twenty-four hours, have killed all the chickens that were inoculated with it, its effect after two, three, or four days considerably diminishes, and an inoculation with it produces nothing more than a slight indisposition in the animal, and one that is never followed by a serious accident. It is then said that the virulence of the microbe is attenuated.
The air is the agent of this transformation that gradually renders the bacteria benign, for in cultures made under the same circumstances as the preceding, but with the absence of air, the activity of these algæ is preserved for days or weeks, and they will then cause death just as surely as they would have done at the end of one day.
What is remarkable is that animals inoculated with the attenuated _micrococcus_ become for a varying length of time refractory to the action of the most formidable parasites of this kind. Mr. Pasteur has discovered two such vaccine viri--one for chicken cholera and the other for charbon. His results have not been accepted without a struggle, and it required nothing less than public experiment in vaccination, both in France and abroad, to convince the incredulous. There are still people at the present time who assert that Mr. Pasteur's process of vaccination has not a great practical range! And yet, here we have the results; more than 400,000 animals have been vaccinated since 1881, and it has been found that the mortality is ten times less in these than in those that have not been vaccinated!