Walker's Creek Presbyterian Church is a historic Presbyterian church and cemetery located near Pearisburg, Giles County, Virginia. The church was built in 1897–1898, and is a one-story, "L"-shaped, frame building in the Gothic Revival style. It features a metal-sheathed gable roof, painted poplar weatherboard siding, a sandstone foundation, and an entry / bell tower. The adjacent church cemetery was established in 1911.
It was listed on the National Register of Historic Places in 2003, with a boundary increase in 2006.