TO VIEW THE CIRCULATION AND EXAMINE THE PARTICLES OF THE BLOOD. The principal part the observer must aim at, in order to view the circulation of the blood, is to procure those small animals or insects that are most transparent, that by seeing through them he may be enabled to discover the internal motion. The particular kinds best adapted for the purpose will be enumerated in the descriptive catalogue at the end of this work.
If a small eel be used for this purpose, it must be cleansed from the slime which covers it; after which it maybe put either in the fish-pan, or a glass tube filled with water, and then placed under the microscope. If the eel be small enough, the circulation may be viewed in the most satisfactory manner. Leeuwenhoeck has given, in his 112th Epistle, an accurate description of the blood vessels in part of the tail of an eel. The same figure may also be seen in my father’s Micrographia Illustrata, fourth edition, Plate XVII. The tail of any other small fish may be applied in the same manner, or tied on a slip of flat glass, and be thus laid before the microscope. Flounders, eels, and gudgeons, are to be had at almost any time in London. N. B. By filling the tube with water, when an eel is used, it will in a great measure prevent the sliminess of the eel from soiling the glass.
To view the particles of the blood, take a small drop of it when warm, and spread it as thin as possible upon a flat piece of glass. By diluting it a little with warm water, some of the larger particles will divide from the smaller, and many of them will be subdivided into still smaller; or a little drop of blood may be put into a capillary tube of glass, and be then presented before the microscope. Mr. Baker advises the mixing the blood with a little warm milk, which he says, will cause the unbroken particles to be very distinctly seen. But the most accurate observer of these particles was Mr. Hewson, and he says they have been termed globules with great impropriety, being in reality flat bodies. When we consider how many ingenious persons have been employed in examining the blood with the best microscopes, it appears surprizing that the figure of the particles should be mistaken; but the wonder is lessened when we reflect how many obvious things are overlooked, till our attention is particularly directed towards them; and besides, the blood in the human subject, and in quadrupeds, is so full of these particles, that it is with great difficulty they can be seen separate, until the blood is diluted. It was by discovering a proper method to effect this, that Mr. Hewson was indebted for his success. He diluted the particles with serum, in which they would remain undissolved, and as he could dilute them to any degree with the serum, he could easily examine the particles distinct from each other; for example, take a small quantity of the serum of the human blood, and shake a piece of crassamentum in it, till it be coloured a little with the red particles; then with a soft hair pencil spread a little of it on a piece of thin glass, and place this glass under the microscope, in such a manner as not to be quite horizontal, but rather higher at one end than the other; by which means the serum will flow from the higher to the lower extremity, and as it flows, some of the particles will be found to swim on their flat sides, and will appear to have a dark spot in the middle; others will turn over from one side to the other, as they roll down the glass.