The 2000 UCLA Bruins softball team represented the University of California, Los Angeles in the 2000 NCAA Division I softball season. The Bruins were coached by Sue Enquist, in her twelfth season as head coach. The Bruins played their home games at Easton Stadium and finished with a record of 46–12–1. They competed in the Pacific-10 Conference, where they finished third with a 14–7 record.
The Bruins were invited to the 2000 NCAA Division I softball tournament, where they swept the Regional and then completed a run to the title game of the Women's College World Series where they fell to champion Oklahoma.