EXP. 3.--On July 16th, at Coalburgh, eggs were obtained from several females, all _Tharos_, as no other form was flying. In four days the eggs hatched; the larval stage was twenty-two, and the pupal stage seven days; but, as before, many larvæ lingered. The first butterfly emerged on August 18th. All were _Tharos_, and none of the larvæ had been lethargic. This was the third generation from the second laying of eggs.
EXP. 4.--On August 15th, at Coalburgh, eggs were obtained from a female _Tharos_, and then taken directly to the Catskill Mountains, where they hatched on the 20th. This was the fourth generation from the third laying of eggs. In Virginia, and during the journey, the weather had been exceedingly warm, but on reaching the mountains it was cool, and at night decidedly cold. September was wet and cold, and the larvæ were protected in a warm room at night and much of the time by day, as they will not feed when the temperature is less than about 8° R. The first pupa was formed September 15th, twenty-six days from the hatching of the larvæ, and others at different dates up to September 26th, or thirty-seven days from the egg. Fifty-two larvæ out of 127 became lethargic after the second moult on September 16th, and on September 26th fully one half of these lethargic larvæ commenced to feed again, and moulted for the third time, after which they became again lethargic and remained in this state. The pupæ from this batch were divided into three portions:--
A. This lot was brought back to Coalburgh on October 15th, the weather during the journey having been cold with several frosty nights, so that for a period of thirty days the pupæ had at no time been exposed to warmth. The butterflies began to emerge on the day of arrival, and before the end of a week all that were living had come forth, viz., 9 males and 10 females. “Of these 9 males 4 were changed to _Marcia_ var. C, 3 were var. D, and 2 were not changed at all. Of the 10 females 8 were changed, 5 of them to var. B, 3 to var. C. The other 2 females were not different from many _Tharos_ of the summer brood, having large discal patches on under side of hind wing, besides the markings common to the summer brood.”